2 edition of Plasmid-determined proteins in Rhizobium Leguminosanum. found in the catalog.
Plasmid-determined proteins in Rhizobium Leguminosanum.
N. J. Dibb
Thesis (Ph.D.) - University of East Anglia, School of Biological Sciences, 1983.
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Journal of General Microbiology (),Printed in Great Britain Plasmid-determined Bacteriocin Production by Rhizobium leguminosarum By PENELOPE R. HIRSCH* John Innes Institute. The conjugative Rhizobium leguminosarum plasmid pJB5JI, which carries genes that determine the ability to nodulate peas, was transferred from R.
leguminosarum strain T3 into R. phaseoli strain Summary. InRhizobium phaseoli strainthe Md plasmid pRP2JI which determines the ability to produce nitrogen-fixing nodules onPhaseolus beans (Nod + Fix +) and the production of melanin on L-tyrosine-containing media (Mel +), was shown to be transmissible by conjugation to otherRhizobium strains.
When pRP2JI was transferred to Nod-strains ofR. leguminosarum (which normally nodulates Cited by: This chapter discusses the plasmids of Rhizobium and symbiotic nitrogen fixation.
The interaction between members of the genus Rhizobium and leguminous plants is specific. Legumes belonging to a given cross-inoculation group can be nodulated effectively only by certain by: Preparation of supernatant proteins.
To visualize bacteriocin protein, the method utilized by Hirsch was ium cultures were grown in ml of TY medium for 5 days. The cultures were pelleted with a GSA rotor at 5, × g for 20 min. The supernatant was poured off and treated with chloroform (10% [vol/vol]) in a separatory by: The antiserum to alkaline phosphate does not react with Rhizobium proteins (lane 1) but does react with several E.
coli proteins (lane 3). The antiserum recognized a protein corresponding to the full-sized NodT-PhoA fusion protein in E. coli [pIJ] (lane 4, arrow), but a smaller protein from RI /pIJ (lane 2), probably Cited by: Summary.
Selection was made for the transposition of the kanamycin resistance transposon Tn5 from a location on the chromosome of R. leguminosarum into a transmissible, bacteriocinogenic plasmid that also carries genes required for the induction of nitrogen-fixing nodules on peas.
One hundred and sixty independent insertions into transmissible plasmids were by: Rhizobium leguminosarum bv. viciae is the specific symbiont of the legumes of the tribe Vicieae which comprises the genera Vicia, Pisum, Lens, and Lathyrus.
Differences in host plant preference for specific rhizobial genotypes within natural populations have been previously reported among these legume genera and species (12, 15, 22, 23).Cited by: Abstract.
This review focuses on the functions of nodulation (nod) genes in the interaction between rhizobia and nod genes are the key bacterial determinants of the signal exchange between the two symbiotic partners. The product of the nodD gene is a transcriptional activator protein that functions as receptor for a flavonoid plant by: Rhizobium sp.
for Lablab, NGRcross-reacted with all of the fast-growing PRC FA's. However, the IF cross-reactivity of NGR was greatest with FA's prepared against USDA and Immunodiffusion analysis The results of immunodiffusion cross-reactions of 33 strains of Rhizobium with somatic and whole cell antisera produced.